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Evaluation of antiviral drugs using recombinant HBoV1-HiBiT NS1. A HEK293T cells were transfected with pHBoV1-HiBiT NS1 in the presence of <t>cidofovir</t> (200 ​μM), favipiravir (50 ​μM), ivermectin (10 ​μM), nevirapine (50 ​μM), remdesivir (10 ​μM), ribavirin (50 ​μM), sofosbuvir (50 ​μM), or zidovudin (50 ​μM). At 72 ​h post-transfection, the Nano-Glo luciferase assay was performed. B, C HEK293T cells were transfected with pHBoV1-HiBiT NS1 in the presence of increasing (2-fold) concentrations of ivermectin. At 72 ​h post-transfection, luciferase activity was measured using the Nano-Glo luciferase assay (B) , while the intracellular DNA copy number was quantified by qPCR (C) . The EC50 and CC50 values were determined by fitting the dose-response curves with four-parameter logistic regression in GraphPad Prism. ∗, P ​< ​0.05, ∗∗∗, P ​< ​0.001; ns, not significant.
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Evaluation of antiviral drugs using recombinant HBoV1-HiBiT NS1. A HEK293T cells were transfected with pHBoV1-HiBiT NS1 in the presence of cidofovir (200 ​μM), favipiravir (50 ​μM), ivermectin (10 ​μM), nevirapine (50 ​μM), remdesivir (10 ​μM), ribavirin (50 ​μM), sofosbuvir (50 ​μM), or zidovudin (50 ​μM). At 72 ​h post-transfection, the Nano-Glo luciferase assay was performed. B, C HEK293T cells were transfected with pHBoV1-HiBiT NS1 in the presence of increasing (2-fold) concentrations of ivermectin. At 72 ​h post-transfection, luciferase activity was measured using the Nano-Glo luciferase assay (B) , while the intracellular DNA copy number was quantified by qPCR (C) . The EC50 and CC50 values were determined by fitting the dose-response curves with four-parameter logistic regression in GraphPad Prism. ∗, P ​< ​0.05, ∗∗∗, P ​< ​0.001; ns, not significant.

Journal: Virologica Sinica

Article Title: Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies

doi: 10.1016/j.virs.2025.03.009

Figure Lengend Snippet: Evaluation of antiviral drugs using recombinant HBoV1-HiBiT NS1. A HEK293T cells were transfected with pHBoV1-HiBiT NS1 in the presence of cidofovir (200 ​μM), favipiravir (50 ​μM), ivermectin (10 ​μM), nevirapine (50 ​μM), remdesivir (10 ​μM), ribavirin (50 ​μM), sofosbuvir (50 ​μM), or zidovudin (50 ​μM). At 72 ​h post-transfection, the Nano-Glo luciferase assay was performed. B, C HEK293T cells were transfected with pHBoV1-HiBiT NS1 in the presence of increasing (2-fold) concentrations of ivermectin. At 72 ​h post-transfection, luciferase activity was measured using the Nano-Glo luciferase assay (B) , while the intracellular DNA copy number was quantified by qPCR (C) . The EC50 and CC50 values were determined by fitting the dose-response curves with four-parameter logistic regression in GraphPad Prism. ∗, P ​< ​0.05, ∗∗∗, P ​< ​0.001; ns, not significant.

Article Snippet: The small-molecule compounds cidofovir (#HY-17438), favipiravir (#HY-14768), ivermectin (#HY-15310), nevirapine (#HY-10570), remdesivir (#HY-104077), ribavirin (#HY-B0434), sofosbuvir (#HY-15005), and zidovudine (#HY-17413) were purchased from MCE.

Techniques: Recombinant, Transfection, Luciferase, Activity Assay